Product name: Pyruvate Assay Kit
Colorimetric / Fluorometric
Urine, serum, plasma, cell culture extracts, other biological fluids, tissue extracts
Test type: Quantitative
Sensitivity: > 1 µM
Distance: 1 µM – 200 µM
Test time: 0h 40m
The ab65342 Pyruvate (Colorimetric / Fluorometric) Assay Kit provides a simple, straightforward, automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media, etc.
In the pyruvate assay protocol, pyruvate oxidase is oxidized by enzymatic reactions to generate color (λ = 570 nm) and fluorescence (at Ex / Em = 535/587 nm). Since the color or intensity of fluorescence is proportional to the pyruvate content, the pyruvate concentration can be accurately measured.
Pyruvate Assay Protocol Summary:
- add samples and standards to wells
- add the reaction mixture and incubate for 30 min at room temperature
- analyze with a microplate reader
Platform: Microplate reader
Store at -20 ° C. See protocols.
Pyruvate is a key intersection molecule in the network of metabolic pathways. Pyruvate can be made from glucose through glycolysis converted back to carbohydrates through gluconeogenesis, or to fatty acids or energy through acetyl CoA. It can also be used to build the amino acid alanine and convert it into ethanol. Glucose is converted to pyruvate which is then converted to acetyl CoA, the main input of the citric acid cycle (TCA). If not enough oxygen is available, pyruvate breaks down anaerobically, creating lactate in animals and ethanol in plants and microorganisms.