Pyruvate Assay Kit 100 Assays

General description

Product name: Pyruvate Assay Kit

Detection method

Colorimetric / Fluorometric

Example type

Urine, serum, plasma, cell culture extracts, other biological fluids, tissue extracts

Test type: Quantitative

Sensitivity: > 1 µM

Distance: 1 µM – 200 µM

Test time: 0h 40m

Product description

The ab65342 Pyruvate (Colorimetric / Fluorometric) Assay Kit provides a simple, straightforward, automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media, etc.

In the pyruvate assay protocol, pyruvate oxidase is oxidized by enzymatic reactions to generate color (λ = 570 nm) and fluorescence (at Ex / Em = 535/587 nm). Since the color or intensity of fluorescence is proportional to the pyruvate content, the pyruvate concentration can be accurately measured.

Pyruvate Assay Protocol Summary:

  • add samples and standards to wells
  • add the reaction mixture and incubate for 30 min at room temperature
  • analyze with a microplate reader

Platform: Microplate reader

Properties

Storage instructions

Store at -20 ° C. See protocols.

Relevance

Pyruvate is a key intersection molecule in the network of metabolic pathways. Pyruvate can be made from glucose through glycolysis converted back to carbohydrates through gluconeogenesis, or to fatty acids or energy through acetyl CoA. It can also be used to build the amino acid alanine and convert it into ethanol. Glucose is converted to pyruvate which is then converted to acetyl CoA, the main input of the citric acid cycle (TCA). If not enough oxygen is available, pyruvate breaks down anaerobically, creating lactate in animals and ethanol in plants and microorganisms.

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