Luteolin induces myelodysplastic syndrome‑derived cell apoptosis through the p53‑dependent mitochondrial signaling pathway mediated by reactive oxygen species.
Luteolin, a typical dietary flavonoid, induces the apoptosis of cells in a number of forms of most cancers. Nevertheless, its function in myelodysplastic syndrome (MDS) and the potential underlying mechanisms stay to be elucidated. To judge the potential profit and underlying mechanisms of luteolin in MDS cells, the viability of SKM‑1 cells and first bone marrow (PBM) mononuclear cells from sufferers with intermediate‑ or excessive‑danger MDS have been assessed utilizing a Cell Counting Package‑eight assay. The apoptotic options of cell morphology have been assessed utilizing Wright‑Giemsa staining, DNA fragmentation was analyzed by agarose gel electrophoresis, and the extent of apoptosis was quantified by move cytometry (FCM).
Reactive oxygen species (ROS) have been measured by FCM with 2,7‑dichlorodihydrofluorescein diacetate staining and mitochondrial membrane potential (ΔΨm) was decided utilizing 5,5′,6,6’‑tetrachloro‑1,1′,3,3’‑tetraethylbenzimidazolylcarbocyanine iodide staining. Caspase exercise was detected utilizing a fluorometric protease assay. Moreover, the consequences of luteolin on the expression of apoptosis‑associated proteins have been analyzed utilizing western blot evaluation.
Luteolin additionally markedly inhibited the proliferation of mononuclear cells from sufferers with intermediate‑ or excessive‑danger MDS. Luteolin suppressed cell proliferation, primarily on account of the induction of apoptosis, as demonstrated by typical apoptotic morphological options, the ladder sample of genomic DNA fragmentation, and the outcomes of FCM utilizing Annexin V‑FITC/PI double staining. It was additionally discovered that brief‑time period publicity of SKM‑1 cells to luteolin led to a marked improve within the accumulation of ROS.
The elevated intracellular stage of ROS appeared to induce the activation of p53 and elevate the B‑cell lymphoma 2 (Bcl‑2)‑related X protein/Bcl‑2 ratio, which modulates ΔΨm and triggers the discharge of cytochrome c, and will improve the actions of apoptotic protease activating issue 1, caspase‑3, ‑eight and ‑9 to additional set off the destruction of structural and particular proteins and thereby cell apoptosis. The ensuing information revealed that luteolin considerably inhibited the proliferation of SKM‑1 cells in vitro, and its half maximal inhibitory focus was 139.41 µM at 24 h and 23.95 µM at 72 h.

Results of low doses Trichlorfon publicity on Rana chensinensis tadpoles.
Trichlorfon is an organophosphate insecticide broadly utilized in aquaculture and agriculture. Little is understood in regards to the results of long-term of low doses trichlorfon publicity on amphibians. On this research, we investigated the consequences of low doses trichlorfon on Rana chensinensis tadpoles after publicity to 0.01, 0.1, and 1.Zero mg/L trichlorfon for two and four weeks. Survival, development, growth and mortality have been monitored frequently over the course of publicity. The outcomes confirmed that trichlorfon led to a lower in tadpole survival. Reductions in development and disruptions to the event of tadpoles have been noticed in trichlorfon therapies.
Morphological abnormalities of affected tadpoles included axial flexures, skeletal malformations and lateral kinks. Trichlorfon elevated the frequency of micronucleus (MN) formation in circulating erythrocytes of tadpoles uncovered for two weeks to 0.1 and 1.Zero mg/L trichlorfon. In any respect concentrations, an enhanced frequency of MN formation was noticed in tadpoles uncovered for four weeks. Publicity to trichlorfon induced different nuclear abnormalities corresponding to lobed and notched nuclei solely in tadpoles uncovered to 1.Zero mg/L trichlorfon for four weeks.
Green One-Step qRT-PCR SuperMix |
abx098038-100l |
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Green Two-Step qRT-PCR SuperMix (GC Rich) |
abx098037-100l |
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abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems |
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MBS402025-4Reactions |
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abx098002-100l |
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abx098002-200l |
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abx098888-100l |
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100 µl |
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Plus PCR SuperMix |
abx098888-200l |
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K5055004 |
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abx098005-100l |
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100 µl |
EUR 262.5 |
T PCR SuperMix (-dye) |
abx098005-200l |
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abx098006-100l |
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AmpHS 2X PCR SuperMix |
MB203-P100 |
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abx098025-100l |
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abx098025-1ml |
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abx098025-200l |
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abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems |
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EUR 727.2 |
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Two-Step RT-PCR SuperMix (15 kb) |
abx098026-100l |
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abx098026-1ml |
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abx098026-200l |
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abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems |
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20-abx098009 |
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FastPfu PCR SuperMix (-dye) |
abx098010-100l |
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100 µl |
EUR 300 |
FastPfu PCR SuperMix (-dye) |
abx098010-200l |
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200 µl |
EUR 575 |
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20-abx098010 |
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PCR SuperMix for PAGE (+dye) |
abx098004-100l |
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PCR SuperMix for PAGE (+dye) |
abx098004-200l |
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200 µl |
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One-Step gDNA Removal and cDNA Synthesis SuperMix |
abx098859-100l |
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100 µl |
EUR 1062.5 |
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One-Step gDNA Removal and cDNA Synthesis SuperMix |
abx098859-1ml |
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1 ml |
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One-Step gDNA Removal and cDNA Synthesis SuperMix |
abx098859-200l |
Abbexa |
200 µl |
EUR 1425 |
One Step MultiPlex qRT PCR Kit with Probe |
9K-005-0012 |
Bio Basic |
100Rxn, 100prep |
EUR 372.16 |
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High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR) |
abx098007-100l |
Abbexa |
100 µl |
EUR 262.5 |
High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR) |
abx098007-200l |
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One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb) |
abx098022-100l |
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One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb) |
abx098022-200l |
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200 µl |
EUR 1325 |
EnTurbo™ SYBR Green PCR SuperMix |
EQ001 |
ELK Biotech |
5mL |
EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix |
EQ001-25mL |
ELK Biotech |
25mL |
EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix |
EQ001-5mL |
ELK Biotech |
5mL |
EUR 80 |
One-Step gDNA Removal and cDNA Synthesis SuperMix (8 kb) |
abx098015-100l |
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1 ml |
EUR 1737.5 |
One-Step gDNA Removal and cDNA Synthesis SuperMix (8 kb) |
abx098015-200l |
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200 µl |
EUR 862.5 |
One-Step gDNA Removal and cDNA Synthesis SuperMix (12 kb) |
abx098017-100l |
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abx098017-1ml |
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1 ml |
EUR 2225 |
One-Step gDNA Removal and cDNA Synthesis SuperMix (12 kb) |
abx098017-200l |
Abbexa |
200 µl |
EUR 962.5 |
HiScript II One Step qRT-PCR Probe Kit |
Q222-01 |
Vazyme |
100 rxn (50 μl/rxn) |
EUR 133.5 |
HiScript II One Step qRT-PCR Probe Kit |
Q223-EN01 |
Vazyme |
250 rxns (20 μl/rxn) |
EUR 157 |
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Q223-EN01-250rxns |
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250 rxns |
EUR 162.57 |
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Q225-EN01 |
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100 rxns |
EUR 94.21 |
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Q225-EN01-100rxns |
Vazyme |
100 rxns |
EUR 97.55 |
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Q225-EN02 |
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1,000 rxns |
EUR 710.17 |
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Q225-EN02-1000rxns |
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EUR 735.38 |
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Q225-EN03 |
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EUR 3261 |
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5,000 rxns |
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Fast-QCell-Eva-One-Step-qRT-PCR-SuperMix-Kit |
K5054002 |
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200 rxn |
EUR 327.6 |
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K5054004 |
Biochain |
400 rxn |
EUR 497.7 |
AmpLong 2X PCR SuperMix (15-30 sec/kb; ≤20kb, 1X) |
MB204-P100 |
Bio-Helix |
100 rxns2 x 1.25 mL |
EUR 13 |
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MB202-P100 |
Bio-Helix |
100 rxns2 x 1.25 mL |
EUR 11 |
HiScript II U+ One Step qRT-PCR Probe Kit |
Q223-01 |
Vazyme |
100 rxn (50 μl/rxn) |
EUR 444 |
HiScript II One Step qRT-PCR SYBR Green Kit |
Q221-00-20rxns |
Vazyme |
20 rxns |
EUR 1.09 |
HiScript II One Step qRT-PCR SYBR Green Kit |
Q221-01 |
Vazyme |
100 rxn (50 μl/rxn) |
EUR 133.5 |
HiScript II One Step qRT-PCR SYBR Green Kit |
Q221-01-250rxns |
Vazyme |
250 rxns |
EUR 138.24 |
All-in-One First-Strand cDNA Synthesis SuperMix for PCR |
abx098023-100l |
Abbexa |
100 µl |
EUR 1050 |
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abx098023-1ml |
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abx098023-200l |
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200 µl |
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abx098023-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 878.4 |
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First-Strand cDNA Synthesis SuperMix for PCR |
abx098018-100l |
Abbexa |
100 µl |
EUR 850 |
First-Strand cDNA Synthesis SuperMix for PCR |
abx098018-1ml |
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1 ml |
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abx098018-200l |
Abbexa |
200 µl |
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First-Strand cDNA Synthesis SuperMix for PCR |
abx098018-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 693.6 |
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EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed) |
EQ014 |
ELK Biotech |
5mL |
EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed) |
EQ014-25mL |
ELK Biotech |
25mL |
EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed) |
EQ014-5mL |
ELK Biotech |
5mL |
EUR 80 |
EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed) |
EQ013 |
ELK Biotech |
5mL |
EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed) |
EQ013-25mL |
ELK Biotech |
25mL |
EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed) |
EQ013-5mL |
ELK Biotech |
5mL |
EUR 80 |
One Step qRT-PCR Kit(一步法荧光定量反转录PCR Kit) |
4305A |
Unibiotest |
50 Tests*20μL |
EUR 100 |
One Step qRT-PCR Kit(一步法荧光定量反转录PCR Kit) |
4305B |
Unibiotest |
125 Tests*20μL |
EUR 200 |
One Step qRT-PCR Kit(一步法荧光定量反转录PCR Kit) |
4305C |
Unibiotest |
250 Tests*20μL |
EUR 350 |
HiScript III One Step qRT-PCR Probe 5 × Master Mix |
Q611-EN01 |
Vazyme |
100 rxns (20 μl/rxn) |
EUR 108.69 |
HiScript III One Step qRT-PCR Probe 5 × Master Mix |
Q611-EN01-100rxns |
Vazyme |
100 rxns |
EUR 112.55 |
HiScript III One Step qRT-PCR Probe 5 × Master Mix |
Q611-EN02 |
Vazyme |
1,000 rxns (20 μl/rxn) |
EUR 905.77 |
HiScript III One Step qRT-PCR Probe 5 × Master Mix |
Q611-EN02-1000rxns |
Vazyme |
1,000 rxns |
EUR 937.93 |
HiScript III One Step qRT-PCR Probe 5 × Master Mix |
Q611-EN03 |
Vazyme |
10,000 rxns (20 μl/rxn) |
EUR 7608.5 |
HiScript III One Step qRT-PCR Probe 5 × Master Mix |
Q611-EN03-10000rxns |
Vazyme |
10,000 rxns |
EUR 7878.6 |
OneScriptcDNA Synthesis SuperMix |
G451 |
ABM |
25 x 20 ul reactions |
EUR 106.8 |
OneScriptcDNA Synthesis SuperMix |
G452 |
ABM |
100 x 20 ul reactions |
EUR 169.2 |
High Fidelity (HiFi) PCR SuperMix (-dye) (lambda DNA, cDNA and plasmid DNA) |
abx098008-100l |
Abbexa |
100 µl |
EUR 262.5 |
High Fidelity (HiFi) PCR SuperMix (-dye) (lambda DNA, cDNA and plasmid DNA) |
abx098008-200l |
Abbexa |
200 µl |
EUR 475 |
High Fidelity (HiFi) PCR SuperMix (-dye) (lambda DNA, cDNA and plasmid DNA) |
20-abx098008 |
Abbexa |
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Probe qPCR SuperMix |
20-abx098040 |
Abbexa |
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OneScriptPlus cDNA Synthesis SuperMix |
G453 |
ABM |
25 x 20 ul reactions |
EUR 116.4 |
OneScriptPlus cDNA Synthesis SuperMix |
G454 |
ABM |
100 x 20 ul reactions |
EUR 202.8 |
All-in-One cDNA Synthesis SuperMix |
B24403 |
Bimake |
200 ractions |
EUR 547.2 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
All-in-One cDNA Synthesis SuperMix |
B24408 |
Bimake |
1000 ractions |
EUR 1743.6 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
Probe Direct SuperMix |
abx461057-1096tests |
Abbexa |
10 × 96 tests |
Ask for price |
Probe Direct SuperMix |
abx461057-596tests |
Abbexa |
5 × 96 tests |
EUR 2800 |
Probe Direct SuperMix |
abx461057-96tests |
Abbexa |
96 tests |
EUR 812.5 |
Evo? cDNA Supermix |
M1168-100 |
Biovision |
each |
EUR 457.2 |
Evo? cDNA Supermix |
M1168-25 |
Biovision |
each |
EUR 320.4 |
Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye |
MB1004 |
BosterBio |
100 reactions |
EUR 239 |
Description: Boster's Green Dye One Step qRT-PCR Master Mix contains all the reagents necessary for reverse transcription and PCR amplification to occur in a single PCR re-action tube, without the template. The Master Mix contains a qRT-PCR Enzyme Mix and a Green Dye qPCR MasterMix, including proprietary Reverse Transcriptase, Ribonuclease Inhibitor, dNTPs and a finely balanced ratio of Oligo (dT)s and Random Primers. The Master Mix also has the high specificity of hot start polymerase. This Master Mix offers the user an efficient and reliable alternative to conventional “two-step” qRT-PCR. Gene-specific primers must be used along with this kit. _x000D__x000D_ More about our proprietary Reverse Transcriptase: _x000D_ The native Reverse Transcriptase has RNase H capacity and degrades mRNA. Using a series of strategic targeted mutations, our scientists successfully nullified the RNase H activity of our RT enzyme, thus preventing RNA degradation during first-strand cDNA synthesis, resulting in higher yields and increase in the achievable length of synthesized cDNA. The engineered Reverse Transcriptase also contains a fidelity‐enhancing sub-unit which ensures superior accuracy in reverse transcription. _x000D_ |
Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye |
MB1004-100Reactions |
BosterBio |
100 Reactions |
EUR 392.4 |
Novo? cDNA Supermix |
M1169-100 |
Biovision |
each |
EUR 529.2 |
Novo? cDNA Supermix |
M1169-25 |
Biovision |
each |
EUR 346.8 |
Green qPCR SuperMix |
abx098031-100l |
Abbexa |
100 µl |
EUR 675 |
Green qPCR SuperMix |
20-abx098031 |
Abbexa |
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Green qPCR SuperMix |
abx098031-200l |
Abbexa |
200 µl |
EUR 912.5 |
cDNA Synthesis SuperMix |
abx098014-100l |
Abbexa |
100 µl |
EUR 725 |
cDNA Synthesis SuperMix |
20-abx09801420ulSystems |
Abbexa |
-
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- 50 rxns × 20 ul Systems
- 100 rxns × 20 ul Systems
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As well as, publicity to trichlorfon inside the 0.01-1.Zero mg/L vary elevated the genetic harm index in hepatic tissues in all therapies. Apoptosis-associated DNA fragmentation in hepatic tissues occurred in a weak ladder-like sample. This research presents proof of low doses trichlorfon results on amphibians, highlighting the properties of this organophosphate insecticide that jeopardize nontarget species uncovered to trichlorfon.