JPH203, a selective L-type amino acid transporter 1 inhibitor, induces mitochondria-dependent apoptosis in Saos2 human osteosarcoma cells.

Most traditional cells specific L-type amino acid transporter 2 (LAT2). Nevertheless, L-type amino acid transporter 1 (LAT1) is extremely expressed in lots of tumor cells and presumed to help their elevated development and proliferation. This research examined the consequences of JPH203, a selective LAT1 inhibitor, on cell development and its mechanism for cell loss of life in Saos2 human osteosarcoma cells. FOB human osteoblastic cells and Saos2 cells expressed LAT1 and LAT2 along with their associating protein 4F2 heavy chain, however the expression of LAT2 within the Saos2 cells was particularly weak.

JPH203 and BCH, a non-selective L-type amino acid transporter inhibitor, potently inhibited L-leucine uptake in Saos2 cells. As anticipated, the intrinsic potential of JPH203 to inhibit L-leucine uptake was much more environment friendly than that of BCH in Saos2 cells. Likewise, JPH203 and BCH inhibited Saos2 cell development with JPH203 being superior to BCH on this regard. Moreover, JPH203 elevated apoptosis charges and shaped DNA ladder in Saos2 cells.

Furthermore, JPH203 activated the mitochondria-dependent apoptotic signaling pathway by upregulating pro-apoptotic elements, corresponding to Unhealthy, Bax, and Bak, and the lively type of caspase-9, and downregulating anti-apoptotic elements, corresponding to Bcl-2 and Bcl-xL. These outcomes counsel that the inhibition of LAT1 exercise through JPH203, which can act as a possible novel anti-cancer agent, results in apoptosis mediated by the mitochondria-dependent intrinsic apoptotic signaling pathway by inducing the intracellular depletion of impartial amino acids important for cell development in Saos2 human osteosarcoma cells.

After remedy with completely different concetrations of gambogenic acid for 72 h, the proliferation of HeLa cells was considerably inhibited, and in a focus dependent method. The power of invasion was decreased with the focus of gambogenic acid elevated, which was detected by Transwell chamber assays in vitro. RT PCR and Western blot demonstrated that gambogenic acid up-regulated the expressions of BAX and E-cadherin and down-regulated the expression of mRNA and protein of BCL-2 and NF-κB.

Luteolin induces myelodysplastic syndrome‑derived cell apoptosis through the p53‑dependent mitochondrial signaling pathway mediated by reactive oxygen species.

Luteolin, a typical dietary flavonoid, induces the apoptosis of cells in a number of forms of most cancers. Nevertheless, its function in myelodysplastic syndrome (MDS) and the potential underlying mechanisms stay to be elucidated. To judge the potential profit and underlying mechanisms of luteolin in MDS cells, the viability of SKM‑1 cells and first bone marrow (PBM) mononuclear cells from sufferers with intermediate‑ or excessive‑danger MDS have been assessed utilizing a Cell Counting Package‑eight assay. The apoptotic options of cell morphology have been assessed utilizing Wright‑Giemsa staining, DNA fragmentation was analyzed by agarose gel electrophoresis, and the extent of apoptosis was quantified by move cytometry (FCM).

Reactive oxygen species (ROS) have been measured by FCM with 2,7‑dichlorodihydrofluorescein diacetate staining and mitochondrial membrane potential (ΔΨm) was decided utilizing 5,5′,6,6’‑tetrachloro‑1,1′,3,3’‑tetraethylbenzimidazolylcarbocyanine iodide staining. Caspase exercise was detected utilizing a fluorometric protease assay. Moreover, the consequences of luteolin on the expression of apoptosis‑associated proteins have been analyzed utilizing western blot evaluation.

Luteolin additionally markedly inhibited the proliferation of mononuclear cells from sufferers with intermediate‑ or excessive‑danger MDS. Luteolin suppressed cell proliferation, primarily on account of the induction of apoptosis, as demonstrated by typical apoptotic morphological options, the ladder sample of genomic DNA fragmentation, and the outcomes of FCM utilizing Annexin V‑FITC/PI double staining. It was additionally discovered that brief‑time period publicity of SKM‑1 cells to luteolin led to a marked improve within the accumulation of ROS.

The elevated intracellular stage of ROS appeared to induce the activation of p53 and elevate the B‑cell lymphoma 2 (Bcl‑2)‑related X protein/Bcl‑2 ratio, which modulates ΔΨm and triggers the discharge of cytochrome c, and will improve the actions of apoptotic protease activating issue 1, caspase‑3, ‑eight and ‑9 to additional set off the destruction of structural and particular proteins and thereby cell apoptosis. The ensuing information revealed that luteolin considerably inhibited the proliferation of SKM‑1 cells in vitro, and its half maximal inhibitory focus was 139.41 µM at 24 h and 23.95 µM at 72 h.

JPH203, a selective L-type amino acid transporter 1 inhibitor, induces mitochondria-dependent apoptosis in Saos2 human osteosarcoma cells.

Results of low doses Trichlorfon publicity on Rana chensinensis tadpoles.

Trichlorfon is an organophosphate insecticide broadly utilized in aquaculture and agriculture. Little is understood in regards to the results of long-term of low doses trichlorfon publicity on amphibians. On this research, we investigated the consequences of low doses trichlorfon on Rana chensinensis tadpoles after publicity to 0.01, 0.1, and 1.Zero mg/L trichlorfon for two and four weeks. Survival, development, growth and mortality have been monitored frequently over the course of publicity. The outcomes confirmed that trichlorfon led to a lower in tadpole survival. Reductions in development and disruptions to the event of tadpoles have been noticed in trichlorfon therapies.

Morphological abnormalities of affected tadpoles included axial flexures, skeletal malformations and lateral kinks. Trichlorfon elevated the frequency of micronucleus (MN) formation in circulating erythrocytes of tadpoles uncovered for two weeks to 0.1 and 1.Zero mg/L trichlorfon. In any respect concentrations, an enhanced frequency of MN formation was noticed in tadpoles uncovered for four weeks. Publicity to trichlorfon induced different nuclear abnormalities corresponding to lobed and notched nuclei solely in tadpoles uncovered to 1.Zero mg/L trichlorfon for four weeks.

QCell-Eva One-Step qRT-PCR SuperMix Kit

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EUR 635

Fast QCell-Eva One-Step qRT-PCR SuperMix Kit

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Probe One-Step qRT-PCR SuperMix

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Green Two-Step qRT-PCR SuperMix

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Green One-Step qRT-PCR SuperMix (GC Rich)

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Probe One-Step qRT-PCR SuperMix (GC Rich)

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Green miRNA Two-Step qRT-PCR SuperMix

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Green Two-Step qRT-PCR SuperMix (GC Rich)

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One-Step BrightGreen qRT-PCR-iCycler

G471-iC 100 rxn (20 ul/rxn)
EUR 189.6

One-Step BrightGreen qRT-PCR-ROX

G471-R 100 rxn (20 ul/rxn)
EUR 189.6

One-Step TaqProbe qRT-PCR-ROX

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EUR 189.6

One-Step TaqProbe qRT-PCR-iCycler

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One Step MultiPlex qRT PCR Kit with Probe

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HiScript II One Step qRT-PCR Probe Kit

Q222-01 100 rxn (50 μl/rxn)
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One-Step BrightGreen qRT-PCR-Low ROX

G471-LR 100 rxn (20 ul/rxn)
EUR 189.6

One-Step BrightGreen qRT-PCR-No Dye

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EUR 189.6

One-Step TaqProbe qRT-PCR-Low ROX

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One-Step TaqProbe qRT-PCR-No Dye

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ExCellenCT One-Step BrightGreen qRT-PCR-iCycler

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ExCellenCT One-Step BrightGreen qRT-PCR-ROX

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ExCellenCT One-Step TaqProbe qRT-PCR-ROX

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ExCellenCT One-Step TaqProbe qRT-PCR-iCycler

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HiScript II One Step qRT-PCR SYBR Green Kit

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HiScript II U+ One Step qRT-PCR Probe Kit

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ExCellenCT One-Step BrightGreen qRT-PCR-Low ROX

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ExCellenCT One-Step BrightGreen qRT-PCR-No Dye

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ExCellenCT One-Step TaqProbe qRT-PCR-Low ROX

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One-Step RT-PCR SuperMix (+dye) (4 kb)

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Two-Step RT-PCR SuperMix (12 kb)

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Two-Step RT-PCR SuperMix (15 kb)

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PCR SuperMix

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  • EUR 326.40
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Pro QPCR SuperMix Kit

K5053200 200 reactions
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Pro QPCR SuperMix Kit

K5053400 400 reactions
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

PCR SuperMix (-dye)

20-abx098002
  • EUR 493.20
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PCR SuperMix (+dye)

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  • EUR 493.20
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Plus PCR SuperMix

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Green-2-Go 1-Step qRT-PCR Mastermix-iCycler

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Green-2-Go 1-Step qRT-PCR Mastermix-Low Rox

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T PCR SuperMix (-dye)

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T PCR SuperMix (+dye)

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EasyPfu PCR SuperMix (-dye)

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FastPfu PCR SuperMix (-dye)

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Pro QPCR SuperMix Kit - ROX premixed

K5056200 200 reactions
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pro QPCR SuperMix Kit - ROX premixed

K5056400 400 reactions
EUR 358.8
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

One Step RT PCR Kit

9K-005-0011 100Rxn, 100prep
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Pospiviroid One-Step PCR kit

Oneq-A546-100R 100T
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Pospiviroid One-Step PCR kit

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Lactobacillus One-Step PCR kit

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Lactobacillus One-Step PCR kit

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Lactobacillus One-Step PCR kit

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Lactococcus One-Step PCR kit

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Lactococcus One-Step PCR kit

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Lactococcus One-Step PCR kit

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Leuconostoc One-Step PCR kit

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Pediococcus One-Step PCR kit

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Halobacillus One-Step PCR kit

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Halobacillus One-Step PCR kit

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Halobacillus One-Step PCR kit

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Enterovirus One-Step PCR kit

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Enterovirus One-Step PCR kit

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Mengovirus One-Step PCR kit

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Mengovirus One-Step PCR kit

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Astrovirus One-Step PCR kit

Oneq-H491-100R 100T
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Astrovirus One-Step PCR kit

Oneq-H491-150R 150T
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Astrovirus One-Step PCR kit

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EUR 1410

Calicivirus One-Step PCR kit

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Calicivirus One-Step PCR kit

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Calicivirus One-Step PCR kit

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Adenovirus One-Step PCR kit

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Adenovirus One-Step PCR kit

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Adenovirus One-Step PCR kit

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Polyomavirus One-Step PCR kit

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Polyomavirus One-Step PCR kit

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Polyomavirus One-Step PCR kit

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Ruminococcus One-Step PCR kit

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EUR 1039.2

Ruminococcus One-Step PCR kit

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Ruminococcus One-Step PCR kit

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Bifidobacterium One-Step PCR kit

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Bifidobacterium One-Step PCR kit

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Bifidobacterium One-Step PCR kit

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Sapovirus One-Step PCR kit

Oneq-H630-100R 100T
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Sapovirus One-Step PCR kit

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Sapovirus One-Step PCR kit

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EUR 1410

Parechovirus One-Step PCR kit

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EUR 1932

Parechovirus One-Step PCR kit

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Parechovirus One-Step PCR kit

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EUR 1410

Metapneumovirus One-Step PCR kit

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EUR 1932

Metapneumovirus One-Step PCR kit

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EUR 2646

Metapneumovirus One-Step PCR kit

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EUR 1410

Entamoeba One-Step PCR kit

Oneq-H683-100D 100T
EUR 1039.2

Entamoeba One-Step PCR kit

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EUR 1177.2

Entamoeba One-Step PCR kit

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EUR 861.6

Acanthamoeba One-Step PCR kit

Oneq-H684-100D 100T
EUR 1039.2

Acanthamoeba One-Step PCR kit

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Acanthamoeba One-Step PCR kit

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EUR 861.6

Echovirus One-Step PCR kit

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EUR 1039.2

Echovirus One-Step PCR kit

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EUR 1177.2

Echovirus One-Step PCR kit

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ranavirus One-Step PCR kit

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EUR 1039.2

ranavirus One-Step PCR kit

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ranavirus One-Step PCR kit

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Ruminococcus One-Step PCR kit

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EUR 896.88
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Ruminococcus One-Step PCR kit

Oneq-V619-150D 150T
EUR 1006.5
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Ruminococcus One-Step PCR kit

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EUR 754.2
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Bifidobacterium One-Step PCR kit

Oneq-V621-100D 100T
EUR 896.88
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

Bifidobacterium One-Step PCR kit

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EUR 1006.5
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

Bifidobacterium One-Step PCR kit

Oneq-V621-50D 50T
EUR 754.2
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

One-Step RT-PCR Kit

G174 100 x 50 ul reactions
EUR 279.6

T-Pro Gel/PCR DNA Purification Maxi Kit (10)

RB94-NEL010 10preps/Kit
EUR 193.2

T-Pro Gel/PCR DNA Purification Mini Kit (100)

RB94-NES100 100preps/Kit
EUR 193.2

T-Pro Gel/PCR DNA Purification Mini Kit (250)

RB94-NES250 250preps/Kit
EUR 266.4

QPCR Kit QRT-PCR Mastermix RNA

MOL6356 EACH
EUR 257.64

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 319.2

One-Step gDNA Removal and cDNA Synthesis SuperMix

20-abx09885920ulSystems
  • EUR 1228.80
  • EUR 895.20
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Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye

MB1004-100Reactions 100 Reactions
EUR 392.4

Green-2-Go 1-Step qRT-PCR Mastermix-Rox (100X20ul Rxn)

QPCR010-R 100Rxn
EUR 287.08

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058200 200 reactions
EUR 253.2
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058400 400 reactions
EUR 384
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

PCR SuperMix for PAGE (+dye)

20-abx098004
  • EUR 493.20
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  • EUR 309.60
  • 15 ml
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MyTaq One-Step RT-PCR Kit

BIO-65048 25 Reactions Ask for price

MyTaq One-Step RT-PCR Kit

BIO-65049 100 Reactions Ask for price

One Step AMV RT-PCR Kit

BS6649 100preps
EUR 477.6

Wallemia sebi One-Step PCR kit

Oneq-A076-100D 100T
EUR 1039.2

Wallemia sebi One-Step PCR kit

Oneq-A076-150D 150T
EUR 1177.2

Wallemia sebi One-Step PCR kit

Oneq-A076-50D 50T
EUR 861.6

Tenacibaculum maritimum One-Step PCR kit

Oneq-A133-100D 100T
EUR 1039.2

Tenacibaculum maritimum One-Step PCR kit

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EUR 1177.2

Tenacibaculum maritimum One-Step PCR kit

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EUR 861.6

Geotrichum candidum One-Step PCR kit

Oneq-A341-100D 100T
EUR 1039.2

Geotrichum candidum One-Step PCR kit

Oneq-A341-150D 150T
EUR 1177.2

Geotrichum candidum One-Step PCR kit

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EUR 861.6

Beggiatoa alba One-Step PCR kit

Oneq-A350-100D 100T
EUR 1039.2

Beggiatoa alba One-Step PCR kit

Oneq-A350-150D 150T
EUR 1177.2

Beggiatoa alba One-Step PCR kit

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EUR 861.6

Flexibacter sp. One-Step PCR kit

Oneq-A485-100D 100T
EUR 1039.2

Flexibacter sp. One-Step PCR kit

Oneq-A485-150D 150T
EUR 1177.2

Flexibacter sp. One-Step PCR kit

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EUR 861.6

Thiothrix sp. One-Step PCR kit

Oneq-A526-100D 100T
EUR 1039.2

Thiothrix sp. One-Step PCR kit

Oneq-A526-150D 150T
EUR 1177.2

Thiothrix sp. One-Step PCR kit

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EUR 861.6

Thysanophora penicilioides One-Step PCR kit

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EUR 1039.2

Thysanophora penicilioides One-Step PCR kit

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EUR 1177.2

Thysanophora penicilioides One-Step PCR kit

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EUR 861.6

Streptococcus dysgalactiae One-Step PCR kit

Oneq-A529-100D 100T
EUR 1039.2

Streptococcus dysgalactiae One-Step PCR kit

Oneq-A529-150D 150T
EUR 1177.2

Streptococcus dysgalactiae One-Step PCR kit

Oneq-A529-50D 50T
EUR 861.6

Aspergillus niger One-Step PCR kit

Oneq-A530-100D 100T
EUR 1039.2

Aspergillus niger One-Step PCR kit

Oneq-A530-150D 150T
EUR 1177.2

Aspergillus niger One-Step PCR kit

Oneq-A530-50D 50T
EUR 861.6

Botrytis cinerea One-Step PCR kit

Oneq-A531-100D 100T
EUR 1039.2

Botrytis cinerea One-Step PCR kit

Oneq-A531-150D 150T
EUR 1177.2

As well as, publicity to trichlorfon inside the 0.01-1.Zero mg/L vary elevated the genetic harm index in hepatic tissues in all therapies. Apoptosis-associated DNA fragmentation in hepatic tissues occurred in a weak ladder-like sample. This research presents proof of low doses trichlorfon results on amphibians, highlighting the properties of this organophosphate insecticide that jeopardize nontarget species uncovered to trichlorfon.

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