JPH203, a selective L-type amino acid transporter 1 inhibitor, induces mitochondria-dependent apoptosis in Saos2 human osteosarcoma cells.

Most traditional cells specific L-type amino acid transporter 2 (LAT2). Nevertheless, L-type amino acid transporter 1 (LAT1) is extremely expressed in lots of tumor cells and presumed to help their elevated development and proliferation. This research examined the consequences of JPH203, a selective LAT1 inhibitor, on cell development and its mechanism for cell loss of life in Saos2 human osteosarcoma cells. FOB human osteoblastic cells and Saos2 cells expressed LAT1 and LAT2 along with their associating protein 4F2 heavy chain, however the expression of LAT2 within the Saos2 cells was particularly weak.

JPH203 and BCH, a non-selective L-type amino acid transporter inhibitor, potently inhibited L-leucine uptake in Saos2 cells. As anticipated, the intrinsic potential of JPH203 to inhibit L-leucine uptake was much more environment friendly than that of BCH in Saos2 cells. Likewise, JPH203 and BCH inhibited Saos2 cell development with JPH203 being superior to BCH on this regard. Moreover, JPH203 elevated apoptosis charges and shaped DNA ladder in Saos2 cells.

Furthermore, JPH203 activated the mitochondria-dependent apoptotic signaling pathway by upregulating pro-apoptotic elements, corresponding to Unhealthy, Bax, and Bak, and the lively type of caspase-9, and downregulating anti-apoptotic elements, corresponding to Bcl-2 and Bcl-xL. These outcomes counsel that the inhibition of LAT1 exercise through JPH203, which can act as a possible novel anti-cancer agent, results in apoptosis mediated by the mitochondria-dependent intrinsic apoptotic signaling pathway by inducing the intracellular depletion of impartial amino acids important for cell development in Saos2 human osteosarcoma cells.

After remedy with completely different concetrations of gambogenic acid for 72 h, the proliferation of HeLa cells was considerably inhibited, and in a focus dependent method. The power of invasion was decreased with the focus of gambogenic acid elevated, which was detected by Transwell chamber assays in vitro. RT PCR and Western blot demonstrated that gambogenic acid up-regulated the expressions of BAX and E-cadherin and down-regulated the expression of mRNA and protein of BCL-2 and NF-κB.

Luteolin induces myelodysplastic syndrome‑derived cell apoptosis through the p53‑dependent mitochondrial signaling pathway mediated by reactive oxygen species.

Luteolin, a typical dietary flavonoid, induces the apoptosis of cells in a number of forms of most cancers. Nevertheless, its function in myelodysplastic syndrome (MDS) and the potential underlying mechanisms stay to be elucidated. To judge the potential profit and underlying mechanisms of luteolin in MDS cells, the viability of SKM‑1 cells and first bone marrow (PBM) mononuclear cells from sufferers with intermediate‑ or excessive‑danger MDS have been assessed utilizing a Cell Counting Package‑eight assay. The apoptotic options of cell morphology have been assessed utilizing Wright‑Giemsa staining, DNA fragmentation was analyzed by agarose gel electrophoresis, and the extent of apoptosis was quantified by move cytometry (FCM).

Reactive oxygen species (ROS) have been measured by FCM with 2,7‑dichlorodihydrofluorescein diacetate staining and mitochondrial membrane potential (ΔΨm) was decided utilizing 5,5′,6,6’‑tetrachloro‑1,1′,3,3’‑tetraethylbenzimidazolylcarbocyanine iodide staining. Caspase exercise was detected utilizing a fluorometric protease assay. Moreover, the consequences of luteolin on the expression of apoptosis‑associated proteins have been analyzed utilizing western blot evaluation.

Luteolin additionally markedly inhibited the proliferation of mononuclear cells from sufferers with intermediate‑ or excessive‑danger MDS. Luteolin suppressed cell proliferation, primarily on account of the induction of apoptosis, as demonstrated by typical apoptotic morphological options, the ladder sample of genomic DNA fragmentation, and the outcomes of FCM utilizing Annexin V‑FITC/PI double staining. It was additionally discovered that brief‑time period publicity of SKM‑1 cells to luteolin led to a marked improve within the accumulation of ROS.

The elevated intracellular stage of ROS appeared to induce the activation of p53 and elevate the B‑cell lymphoma 2 (Bcl‑2)‑related X protein/Bcl‑2 ratio, which modulates ΔΨm and triggers the discharge of cytochrome c, and will improve the actions of apoptotic protease activating issue 1, caspase‑3, ‑eight and ‑9 to additional set off the destruction of structural and particular proteins and thereby cell apoptosis. The ensuing information revealed that luteolin considerably inhibited the proliferation of SKM‑1 cells in vitro, and its half maximal inhibitory focus was 139.41 µM at 24 h and 23.95 µM at 72 h.

JPH203, a selective L-type amino acid transporter 1 inhibitor, induces mitochondria-dependent apoptosis in Saos2 human osteosarcoma cells.

Results of low doses Trichlorfon publicity on Rana chensinensis tadpoles.

Trichlorfon is an organophosphate insecticide broadly utilized in aquaculture and agriculture. Little is understood in regards to the results of long-term of low doses trichlorfon publicity on amphibians. On this research, we investigated the consequences of low doses trichlorfon on Rana chensinensis tadpoles after publicity to 0.01, 0.1, and 1.Zero mg/L trichlorfon for two and four weeks. Survival, development, growth and mortality have been monitored frequently over the course of publicity. The outcomes confirmed that trichlorfon led to a lower in tadpole survival. Reductions in development and disruptions to the event of tadpoles have been noticed in trichlorfon therapies.

Morphological abnormalities of affected tadpoles included axial flexures, skeletal malformations and lateral kinks. Trichlorfon elevated the frequency of micronucleus (MN) formation in circulating erythrocytes of tadpoles uncovered for two weeks to 0.1 and 1.Zero mg/L trichlorfon. In any respect concentrations, an enhanced frequency of MN formation was noticed in tadpoles uncovered for four weeks. Publicity to trichlorfon induced different nuclear abnormalities corresponding to lobed and notched nuclei solely in tadpoles uncovered to 1.Zero mg/L trichlorfon for four weeks.

QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054400 400 reactions
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Fast QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054002 200 rxn
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Probe One-Step qRT-PCR SuperMix

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Green One-Step qRT-PCR SuperMix

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Probe One-Step qRT-PCR SuperMix (GC Rich)

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Green One-Step qRT-PCR SuperMix (GC Rich)

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Green Two-Step qRT-PCR SuperMix

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Green miRNA Two-Step qRT-PCR SuperMix

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Green Two-Step qRT-PCR SuperMix (GC Rich)

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PCR SuperMix

20-abx098887
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One-Step RT-PCR SuperMix (+dye) (4 kb)

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One-Step RT-PCR SuperMix (+dye) (8 kb)

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One-Step RT-PCR SuperMix (+dye) (8 kb)

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PCR SuperMix (+dye)

20-abx098003
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PCR SuperMix (-dye)

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Plus PCR SuperMix

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Pro QPCR SuperMix Kit

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Pro QPCR SuperMix Kit

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T PCR SuperMix (-dye)

20-abx098005
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T PCR SuperMix (+dye)

20-abx098006
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EasyPfu PCR SuperMix (-dye)

20-abx098009
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FastPfu PCR SuperMix (-dye)

20-abx098010
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Two-Step RT-PCR SuperMix (12 kb)

abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)
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Two-Step RT-PCR SuperMix (15 kb)

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PCR SuperMix for PAGE (+dye)

20-abx098004
  • EUR 493.20
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Pro QPCR SuperMix Kit - ROX premixed

K5056200 200 reactions
EUR 161

Pro QPCR SuperMix Kit - ROX premixed

K5056400 400 reactions
EUR 291

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058200 200 reactions
EUR 188

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058400 400 reactions
EUR 316

One-Step gDNA Removal and cDNA Synthesis SuperMix

20-abx09885920ulSystems
  • EUR 1228.80
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High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)

20-abx098007
  • EUR 260.40
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One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb)

20-abx09802220ulSystems
  • EUR 1128.00
  • EUR 828.00
  • 100 rxns × 20 ul Systems
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One Step MultiPlex qRT PCR Kit with Probe

9K-005-0012 100Rxn, 100prep
EUR 372.16

One-Step gDNA Removal and cDNA Synthesis SuperMix (8 kb)

20-abx09801520ulSystems
  • EUR 693.60
  • EUR 594.00
  • 100 rxns × 20 ul Systems
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One-Step gDNA Removal and cDNA Synthesis SuperMix (12 kb)

20-abx09801720ulSystems
  • EUR 794.40
  • EUR 644.40
  • 100 rxns × 20 ul Systems
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Eva QPCR SuperMix Kit

K5052200 200 reactions
EUR 188

Eva QPCR SuperMix Kit

K5052400 400 reactions
EUR 316

HiScript II One Step qRT-PCR Probe Kit

Q222-01 100 rxn (50 μl/rxn)
EUR 396

HiScript II One Step qRT-PCR Probe Kit

Q223-EN01 250 rxns (20 μl/rxn)
EUR 125.6

First-Strand cDNA Synthesis SuperMix for PCR

abx098018-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 693.6

HiScript III One Step qRT-PCR Probe Kit

Q225-EN01 100 rxns
EUR 75.37

HiScript III One Step qRT-PCR Probe Kit

Q225-EN02 1,000 rxns
EUR 568.14

HiScript III One Step qRT-PCR Probe Kit

Q225-EN03 5,000 rxns
EUR 2608.8

Fast Eva QPCR SuperMix Kit

K5052002 200 rxn
EUR 188

Power Eva QPCR SuperMix Kit

K5057200 200 reactions
EUR 161

Power Eva QPCR SuperMix Kit

K5057400 400 reactions
EUR 316

All-in-One First-Strand cDNA Synthesis SuperMix for PCR

abx098023-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 878.4

HiScript II U+ One Step qRT-PCR Probe Kit

Q223-01 100 rxn (50 μl/rxn)
EUR 444

OneScriptcDNA Synthesis SuperMix

G451 25 x 20 ul reactions
EUR 106.8

OneScriptcDNA Synthesis SuperMix

G452 100 x 20 ul reactions
EUR 169.2

HiScript II One Step qRT-PCR SYBR Green Kit

Q221-01 100 rxn (50 μl/rxn)
EUR 396

Probe qPCR SuperMix

20-abx098040
  • EUR 1245.60
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Evo? cDNA Supermix

M1168-100 each
EUR 457.2

Evo? cDNA Supermix

M1168-25 each
EUR 320.4

Novo? cDNA Supermix

M1169-100 each
EUR 529.2

Novo? cDNA Supermix

M1169-25 each
EUR 346.8

Green qPCR SuperMix

20-abx098031
  • EUR 1245.60
  • EUR 526.80
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  • 15 ml
  • 1 ml
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OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 116.4

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 202.8

High Fidelity (HiFi) PCR SuperMix (-dye) (lambda DNA, cDNA and plasmid DNA)

20-abx098008
  • EUR 260.40
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cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 678.00
  • EUR 577.20
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Green qPCR SuperMix UDG

20-abx098032
  • EUR 1362.00
  • EUR 543.60
  • EUR 794.40
  • 15 ml
  • 1 ml
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Top Green qPCR SuperMix

20-abx098033
  • EUR 994.80
  • EUR 260.40
  • EUR 1362.00
  • EUR 493.20
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Tip Green qPCR SuperMix

20-abx098034
  • EUR 1161.60
  • EUR 276.00
  • EUR 1596.00
  • EUR 560.40
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

All-in-One cDNA Synthesis SuperMix

B24403 200 ractions
EUR 547.2
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix

B24408 1000 ractions
EUR 1743.6
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

Library Amplification SuperMix

20-abx098894
  • EUR 309.60
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HiScript III One Step qRT-PCR Probe 5 × Master Mix

Q611-EN01 100 rxns (20 μl/rxn)
EUR 86.95

HiScript III One Step qRT-PCR Probe 5 × Master Mix

Q611-EN02 1,000 rxns (20 μl/rxn)
EUR 724.62

HiScript III One Step qRT-PCR Probe 5 × Master Mix

Q611-EN03 10,000 rxns (20 μl/rxn)
EUR 6086.8

cDNA Synthesis SuperMix for qPCR

20-abx09801920ulSystems
  • EUR 828.00
  • EUR 678.00
  • 100 rxns ×20 ul Systems
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Top Green qPCR SuperMix (+Dye II)

20-abx098890
  • EUR 994.80
  • EUR 260.40
  • EUR 1362.00
  • EUR 493.20
  • 15 ml
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  • 25 ml
  • 5 ml

Tip Green qPCR SuperMix (+Dye II)

20-abx098891
  • EUR 1161.60
  • EUR 276.00
  • EUR 1596.00
  • EUR 560.40
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Library Quantification qPCR SuperMix

20-abx098896
  • EUR 276.00
  • EUR 493.20
  • 1 ml
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HiScript II Q RT SuperMix for qPCR

R222-01 100 rxn (20 μl/rxn)
EUR 282

Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye

MB1004-100Reactions 100 Reactions
EUR 392.4

Fly First-Strand cDNA Synthesis SuperMix

20-abx09885820ulSystems
  • EUR 861.60
  • EUR 693.60
  • 100 rxns × 20 ul Systems
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cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 861.6

miRNA First-Strand cDNA Synthesis SuperMix

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EUR 878.4

HiScript III All-in-one RT SuperMix Perfect for qPCR

R333-01 100 rxns (21 μl/rxn)
EUR 174

HiScript II Q Select RT SuperMix for qPCR

R232-01 100 rxn (20 μl/rxn)
EUR 282

HiScript III RT SuperMix for qPCR (+g DNA wiper)

R323-01 100 rxn (20 μl/rxn)
EUR 351.6

One-Step TaqProbe qRT-PCR-ROX

G493-P 100 rxn (20 ul/rxn)
EUR 189.6

One-Step BrightGreen qRT-PCR-ROX

G471-R 100 rxn (20 ul/rxn)
EUR 189.6

One-Step TaqProbe qRT-PCR-iCycler

G493-PC 100 rxn (20 ul/rxn)
EUR 189.6

One-Step BrightGreen qRT-PCR-iCycler

G471-iC 100 rxn (20 ul/rxn)
EUR 189.6

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
  • EUR 744.00
  • EUR 627.60
  • 100 rxns × 20 ul Systems
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First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
  • EUR 1045.20
  • EUR 794.40
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  • 50 rxns × 20 ul Systems

HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper)

R233-01 100 rxn (20 μl/rxn)
EUR 292.8

One Step RT PCR Kit

9K-005-0011 100Rxn, 100prep
EUR 321

One-Step TaqProbe qRT-PCR-No Dye

G493-PS 100 rxn (20 ul/rxn)
EUR 189.6

One-Step TaqProbe qRT-PCR-Low ROX

G493-PL 100 rxn (20 ul/rxn)
EUR 189.6

One-Step BrightGreen qRT-PCR-No Dye

G471-S 100 rxn (20 ul/rxn)
EUR 189.6

One-Step BrightGreen qRT-PCR-Low ROX

G471-LR 100 rxn (20 ul/rxn)
EUR 189.6

ExCellenCT One-Step TaqProbe qRT-PCR-ROX

G918-P 25 Preps 100 x 20 ul reactions
EUR 212.4

Green-2-Go 1-Step qRT-PCR Mastermix-iCycler

QPCR011-iC 100Rxn
EUR 287.08

ExCellenCT One-Step BrightGreen qRT-PCR-ROX

G917-R 25 Preps 100 x 20 ul reactions
EUR 212.4

ExCellenCT One-Step TaqProbe qRT-PCR-iCycler

G918-PC 25 Preps 100 x 20 ul reactions
EUR 212.4

ExCellenCT One-Step BrightGreen qRT-PCR-iCycler

G917-iC 25 Preps 100 x 20 ul reactions
EUR 212.4

T-Pro Gel/PCR DNA Purification Mini Kit (100)

RB94-NES100 100preps/Kit
EUR 193.2

T-Pro Gel/PCR DNA Purification Mini Kit (250)

RB94-NES250 250preps/Kit
EUR 266.4

T-Pro Gel/PCR DNA Purification Maxi Kit (10)

RB94-NEL010 10preps/Kit
EUR 193.2

Green-2-Go 1-Step qRT-PCR Mastermix-Low Rox

QPCR012-LR 100Rxn
EUR 287.08

ExCellenCT One-Step TaqProbe qRT-PCR-No Dye

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EUR 212.4

ExCellenCT One-Step TaqProbe qRT-PCR-Low ROX

G918-PL 25 Preps 100 x 20 ul reactions
EUR 212.4

ExCellenCT One-Step BrightGreen qRT-PCR-No Dye

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EUR 212.4

ExCellenCT One-Step BrightGreen qRT-PCR-Low ROX

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EUR 212.4

Green-2-Go 1-Step qRT-PCR Mastermix-Rox (100X20ul Rxn)

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One Step RT PCR Kit (cDNA Synthesis + PCR In One Reaction)

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Green-2-Go 1-Step qRT-PCR Mastermix-No Dye (100X20ul Rxn)

QPCR007-NODYE 100Rxn
EUR 287.08

One Step AMV RT-PCR Kit

BS6649 100preps
EUR 477.6

AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE)

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EUR 208.69

AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE)

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EUR 811.58

AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE)

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EUR 6956.4

One Step M-MULV RT-PCR Kit

BS665 100preps
EUR 477.6

HiScipt II One Step RT-PCR Kit

P611-01 50 rxn (50 μl/rxn)
EUR 234

Green-2-Go 1-Step TaqProbe qRT-PCR Mastermix-no Dye (100X20ul Rxn)

QPCR008-NODYE 100Rxn
EUR 341.88

igScript One Step RT-PCR Kit - 100 Reactions

4211 1/EA
EUR 284.4

igScript One Step RT-PCR Kit - 500 Reactions

4213 1/EA
EUR 908.4

T-Pro BCA Protein Assay kit

JB04-D001 500assay/KIT
EUR 244.8

HiScipt II One Step RT-PCR Kit (Dye Plus)

P612-01 50 rxn (50 μl/rxn)
EUR 234

T-Pro Bradford Protein Assay kit(1X)

JB04-D002 500ml/BT
EUR 193.2

T-Pro Plasmid Mini Kit (100)

RB94-YPD100 100preps/Kit
EUR 193.2

T-Pro Plasmid Mini Kit (250)

RB94-YPD250 250preps/Kit
EUR 266.4

T-Pro Plasmid Midi Kit (20)

RB94-YPI020 20preps/Kit
EUR 255.6

T-Pro Plasmid Maxi Kit (10)

RB94-YPM010 10preps/Kit
EUR 266.4

T-Pro Genomic DNA Mini Kit (100)

RB94-NGS100 100preps/kit
EUR 255.6

T-Pro Genomic DNA Midi Kit (20)

RB94-NGM020 20preps/kit
EUR 266.4

T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit)

JT96-K002M 250ml*2/Kit
EUR 244.8

T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit)

JT96-K002S 100ml*2/Kit
EUR 182.4

T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)

JT96-K004M 250ml*2/Kit
EUR 286.8

T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)

JT96-K004S 100ml*2/Kit
EUR 204

T-Pro Plant Genomic DNA Mini Kit (100)

RB94-PGS100 100preps/kit
EUR 266.4

T-Pro Plant Genomic DNA Midi Kit (20)

RB94-PGM020 20preps/kit
EUR 266.4

T-Pro Endotoxin Removal Plasmid Midi kit (25)

RB94-EPI020 20preps/Kit
EUR 297.6

T-Pro Endotoxin Removal Plasmid Maxi kit (10)

RB94-EPM010 10preps/Kit
EUR 318

ranavirus One-Step PCR kit

Oneq-V337-100D 100T
EUR 1039.2

ranavirus One-Step PCR kit

Oneq-V337-150D 150T
EUR 1177.2

ranavirus One-Step PCR kit

Oneq-V337-50D 50T
EUR 861.6

Sapovirus One-Step PCR kit

Oneq-H630-100R 100T
EUR 1932

Sapovirus One-Step PCR kit

Oneq-H630-150R 150T
EUR 2646

Sapovirus One-Step PCR kit

Oneq-H630-50R 50T
EUR 1410

Entamoeba One-Step PCR kit

Oneq-H683-100D 100T
EUR 1039.2

Entamoeba One-Step PCR kit

Oneq-H683-150D 150T
EUR 1177.2

Entamoeba One-Step PCR kit

Oneq-H683-50D 50T
EUR 861.6

Echovirus One-Step PCR kit

Oneq-H722-100D 100T
EUR 1039.2

Echovirus One-Step PCR kit

Oneq-H722-150D 150T
EUR 1177.2

Echovirus One-Step PCR kit

Oneq-H722-50D 50T
EUR 861.6

Mengovirus One-Step PCR kit

Oneq-H453-100R 100T
EUR 1932

Mengovirus One-Step PCR kit

Oneq-H453-150R 150T
EUR 2646

Mengovirus One-Step PCR kit

Oneq-H453-50R 50T
EUR 1410

Astrovirus One-Step PCR kit

Oneq-H491-100R 100T
EUR 1932

Astrovirus One-Step PCR kit

Oneq-H491-150R 150T
EUR 2646

Astrovirus One-Step PCR kit

Oneq-H491-50R 50T
EUR 1410

Adenovirus One-Step PCR kit

Oneq-H501-100D 100T
EUR 1039.2

Adenovirus One-Step PCR kit

Oneq-H501-150D 150T
EUR 1177.2

Adenovirus One-Step PCR kit

Oneq-H501-50D 50T
EUR 861.6

Pospiviroid One-Step PCR kit

Oneq-A546-100R 100T
EUR 1932

Pospiviroid One-Step PCR kit

Oneq-A546-150R 150T
EUR 2646

Pospiviroid One-Step PCR kit

Oneq-A546-50R 50T
EUR 1410

Lactococcus One-Step PCR kit

Oneq-A567-100D 100T
EUR 1039.2

Lactococcus One-Step PCR kit

Oneq-A567-150D 150T
EUR 1177.2

As well as, publicity to trichlorfon inside the 0.01-1.Zero mg/L vary elevated the genetic harm index in hepatic tissues in all therapies. Apoptosis-associated DNA fragmentation in hepatic tissues occurred in a weak ladder-like sample. This research presents proof of low doses trichlorfon results on amphibians, highlighting the properties of this organophosphate insecticide that jeopardize nontarget species uncovered to trichlorfon.

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